IL 7 Protein (OPPA00519)

Catalog No: OPPA00519 (Formerly GWB-3B1505)

Size:2UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized Interleukin -7 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Biological Activity: The ED50 as determined by the dose-dependant stimulation of murine IXN/2B cells is < 0.5ng/ml, corresponding to a Specific Activity of 2 x 10⁶ IU/mg.
  Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.418 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard.
  Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.418 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard.
  Product Introduction: IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.
  Product Description: Interleukin-7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17412 Dalton. The IL-7 is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL7 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Peptide Sequence: MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH.

Target Info

• Gene Symbol: IL7
• Alias Symbols: IL-7
• NCBI Gene Id: 3574
• Protein Name: Interleukin-7
• Description of Target: Recombinant Human Interleukin-7
• Uniprot ID: P13232
• Protein Accession #: NP_000871.1
• Protein Size (# AA): Recombinant