IL-7 Polyclonal Antibody (OABB00421)

Catalog No: OABB00421 (Formerly GWB-BBP344)

Size:100UG

Lane 1: Recombinant Mouse IL-7 Protein 10ng
Lane 2: Recombinant Mouse IL-7 Protein 5ng
Lane 3: Recombinant Mouse IL-7 Protein 2.5ng

IHC(P): Mouse Spleen Tissue

Product Info

• Tested Species Reactivity: Mouse
• Predicted Species Reactivity: Hamster|Human|Mouse
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Interleukin 7 (IL-7) is a protein that in humans is encoded by the IL7 gene. The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. And it is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. In addition, this cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Mouse
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Mouse
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Mouse: By Heat
• Reference: 1. O'Connor, A. M., Crawley, A. M., Angel, J. B.Interleukin-7 enhances memory CD8+ T-cell recall
  responses in health but its activity is impaired in human immunodeficiency virus
  infection.Immunology 131: 525-536, 2010.
  2. Sutherland, G. R., Baker, E., Fernandez, K. E. W., Callen, D. F., Goodwin, R. G., Lupton, S., Namen,
  A. E., Shannon, M. F., Vadas, M. A.The gene for human interleukin 7 (IL7) is at 8q12-13.Hum. Genet.
  82: 371-372, 1989.
  3. Watanabe, M., Ueno, Y., Yajima, T., Iwao, Y., Tsuchiya, M., Ishikawa, H., Aiso, S., Hibi, T., Ishii,
  H.Interleukin 7 is produced by human intestinal epithelial cells and regulates the proliferation of
  intestinal mucosal lymphocytes.J. Clin. Invest. 95: 2945-2953, 1995.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Interleukin-7(IL7) detection. Tested with WB, IHC-P in Mouse.

Target Info

• Gene Symbol: Il7
• Gene Full Name: interleukin 7
• Alias Symbols: A630026I06Rik;hlb36;hlb368;Il;Il-7;interleukin-7.
• NCBI Gene Id: 16196
• Protein Name: Interleukin-7
• Description of Target: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.
• Uniprot ID: P10168
• Molecular Weight: 17727 MW