IL 3 Protein (OPPA00633)

Catalog No: OPPA00633 (Formerly GWB-27A560)

Size:2UG

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Biological Activity: The ED50 as determined by the dose-dependant stimulation of murine M-NFS-60 cells is < 0.05 ng/ml, corresponding to a Specific Activity of 2 x 10⁷ IU/mg.
  Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.154 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL3 as a Reference Standard.
  Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.154 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL3 as a Reference Standard.
  Product Introduction: Interleukin-3 is a pleiotropic cytokine produced primarily by activated T cells. IL-3 is thought to function via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines. IL-3 has also been shown to affect the functional activity of a variety of other cell types including mast cells, eosinophils, megakaryocytes and basophils.
  Product Description: Interleukin-3 mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 15100 Dalton. The IL-3 is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL-3 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Peptide Sequence: The sequence of the first five N - terminal amino acids was determined and was found to be Met - Asp - Thr - His - Arg.

Target Info

• Gene Symbol: IL3
• Alias Symbols: B, P, Il, BPA, PSF, Csfm, HCGF, Il-3, MCGF, Csfmu
• NCBI Gene Id: 16187
• Protein Name: Interleukin-3
• Description of Target: Recombinant Mouse Interleukin-3
• Uniprot ID: P01586
• Protein Accession #: NP_034686.2
• Protein Size (# AA): Recombinant