IL3 ELISA Kit (Dog) (OKCD02637)

Catalog No: OKCD02637

Size:96WELLS

Dog IL3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Canis lupus|Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-104%	84-95%	81-92%	78-95%
  EDTA Plasma (n=5)	96-105%	89-103%	86-96%	92-99%
  Heparin Plasma (n=5)	93-102%	96-103%	80-99%	80-89%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 3 (IL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 3 (IL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 3 (IL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 3 (IL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	83-101	86
  EDTA Plasma (n=5)	83-93	89
  Heparin Plasma(n=5)	98-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 3 (IL3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 3 (IL3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IL3 Microplate: 96 Wells (12 x 8 Well strips)
  • IL3 Lyophilized Standard: 2
  • 100X Biotinylated IL3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 6.5 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 3 (IL3). No significant cross-reactivity or interference between Interleukin 3 (IL3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL3
• Gene Full Name: interleukin 3
• Alias Symbols: hematopoietic growth factor;IL-3;interleukin 3 (colony-stimulating factor, multiple);interleukin-3;mast cell growth factor;MCGF;multipotential colony-stimulating factor;P-cell-stimulating factor.
• NCBI Gene Id: 481497
• Protein Name: Interleukin-3
• Description of Target: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
• Uniprot ID: Q9BDX4
• Protein Accession #: NP_001013857.1
• Nucleotide Accession #: NM_001013835.1
• Protein Size (# AA): 143