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IL-2 ELISA Kit (Mouse) (OKBB00180)

96 Tests
$390.00
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Application:
ELISA
Gene Symbol:
IL-2
Official Gene Full Name:
interleukin 2
NCBI Gene Id:
16183
Alias Symbols:
Interleukin-2, Interleukin 2, IL 2
Description of Target:
Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression.1 IL-2 can act as a growth hormone for both B and T lymphocytes.2 The human gene for interleukin 2 (IL2) is assigned to chromosome 4.3 Mouse IL-2 contains 149 amino acids in mature form with the molecular mass of 17.2KDa.
Protein Name:
Interleukin-2
Swissprot Id:
P04351
Protein Accession #:
NP_032392.1
Nucleotide Accession #:
NM_008366.3
Sample Type:
cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Sensitivity:
< 1 pg/ml
Kit Range:
15.6 pg/ml-1,000 pg/ml
Kit Reproducibility:
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (pg/mL)8121559086193575
St. Dev.4.53611.6125.375.24613.3229.9
%CV5.65.44.36.16.905.2
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology IL-2 ELISA Kit (Mouse) (OKBB00180) is based on standard sandwich enzyme-linked immune-sorbent assay technology. A rat monoclonal antibody specific for IL-2 has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (E.coli,A21-Q169) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL-2 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Mouse IL-2 captured in well.
Kit Component:
ComponentAmount
Lyophilized recombinant mouse IL-2 standard10 ng/tube x 2
Anti-mouse IL-2 Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti- mouse IL-2 antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL-2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL-2.
Predicted Homology Based on Immunogen Sequence:
No detectable cross-reactivity with any other cytokine.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Target Reference:
1. Hollander, G. A.; Zuklys, S.; Morel, C.; Mizoguchi, E.; Mobisson, K.; Simpson, S.; Terhorst, C.; Wishart, W.; Golan, D.
E.; Bhan, A. K.; Burakoff, S. J. Monoallelic expression of the interleukin-2 locus. Science 279: 2118-2121, 1998.
2. Lowenthal, J. W.; Zubler, R. H.; Nabholz, M.; MacDonald, H. R. Similarities between interleukin-2 receptor number
and affinity on activated B and T lymphocytes. Nature 315: 669-672, 1985.
3. Shows, T.; Eddy, R.; Haley, L.; Byers, M.; Henry, M.; Fujita, T.; Matsui, H.; Taniguchi, T. Interleukin 2 (IL2) is
assigned to human chromosome 4. Somat. Cell Molec. Genet. 10: 315-318, 1984.
Specificity:
There is no detectable cross-reactivity with other relevant proteins.
Publications:

Kloog, Y. & Mor, A. Cytotoxic-T-lymphocyte antigen 4 receptor signaling for lymphocyte adhesion is mediated by C3G and Rap1. Mol. Cell. Biol. 34, 978-88 (2014). ELISA, Mouse 24396067

Additional Information:
Range: 15.6pg/ml-1000pg/ml
:::
Principle: Aviva’s mouse IL-2 ELISA Kit was based on standard sandwich
enzyme-linked immune-sorbent assay technology. Mouse IL-2 specific-specific
monoclonal antibodies were precoated onto 96-well plates. The mouse specific
detection monoclonal antibodies were biotinylated. The test samples and
biotinylated detection antibodies were added to the wells subsequently and
then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase
Complex was added and unbound conjugates were washed away with PBS or
TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction.
TMB was catalyzed by HRP to produce a blue color product that changed into
yellow after adding acidic stop solution. The density of yellow is proportional
to the mouse IL-2 amount of sample captured in plate.
:::
Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression.1 IL-2 can act as a growth hormone for both B and T lymphocytes.2 The human gene for interleukin 2 (IL2) is assigned to chromosome 4.3 Mouse IL-2 contains 149 amino acids in mature form with the molecular mass of 17.2KDa.
:::
1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.

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