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Catalog No: OKBB00181
Size:96 Tests
Price: $583.00
SKU
OKBB00181
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's rat IL-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-2 has been precoated onto 96-well plates. Standards (E.coli,A21-Q155) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat IL-2 amount of sample captured in plate.
ELISA Kit Range31.2 pg/ml - 2,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (pg/mL)102761146498.77721345
St. Dev.4.1822.0751.24857.1383.39
%CV4.092.903.58.107.406.2
ELISA Kit Component
ComponentAmount
Lyophilized recombinant rat IL-2 standard10 ng/tube x 2
Anti-rat IL-2 Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti-rat IL-2 antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Additional InformationRange: 31.2pg/ml-2000pg/ml
::Principle: Aviva’s rat IL-2 ELISA Kit was based on standard sandwich enzyme-linked
immune-sorbent assay technology. Rat IL-2 specific-specific monoclonal
antibodies were precoated onto 96-well plates. The rat specific detection
monoclonal antibodies were biotinylated. The test samples and biotinylated
detection antibodies were added to the wells subsequently and then followed
by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was
added and unbound conjugates were washed away with PBS or TBS buffer.
HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was
catalyzed by HRP to produce a blue color product that changed into yellow
after adding acidic stop solution. The density of yellow is proportional to the
rat IL-2 amount of sample captured in plate.
::Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression.1 IL-2 can act as a growth hormone for both B and T lymphocytes.2 The human gene for interleukin 2 (IL2) is assigned to chromosome 4.3
::1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample Typecell culture supernates, serum and plasma (heparin, EDTA, citrate)
Sensitivity<1 pg/ml
Predicted Homology Based on Immunogen SequenceNo detectable cross-reactivity with any other cytokine.
Reference1. Hollander, G. A.; Zuklys, S.; Morel, C.; Mizoguchi, E.; Mobisson, K.; Simpson, S.; Terhorst, C.; Wishart, W.; Golan, D.
E.; Bhan, A. K.; Burakoff, S. J. Monoallelic expression of the interleukin-2 locus. Science 279: 2118-2121, 1998.
2. Lowenthal, J. W.; Zubler, R. H.; Nabholz, M.; MacDonald, H. R. Similarities between interleukin-2 receptor number
and affinity on activated B and T lymphocytes. Nature 315: 669-672, 1985.
3. Shows, T.; Eddy, R.; Haley, L.; Byers, M.; Henry, M.; Fujita, T.; Matsui, H.; Taniguchi, T. Interleukin 2 (IL2) is
assigned to human chromosome 4. Somat. Cell Molec. Genet. 10: 315-318, 1984.
SpecificityNatural and recombinant rat IL-2
ImmunogenExpression system for standard: E.coli; Immunogen sequence: A21-Q155
DescriptionFor quantitative detection of rat IL-2 in cell culture supernatants, serum and plasma(heparin, EDTA, citrate).
Gene SymbolIL2
Gene Full Nameinterleukin 2
Alias SymbolsAldesleukin, IL 2, IL-2, IL2, IL2_HUMAN, Interleukin 2, Interleukin-2, Interleukin2, Involved in regulation of T cell clonal expansion, Lymphokine, T cell growth factor, T-cell growth factor, TCGF
NCBI Gene Id116562
Protein NameInterleukin-2
Description of TargetInterleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression.1 IL-2 can act as a growth hormone for both B and T lymphocytes.2 The human gene for interleukin 2 (IL2) is assigned to chromosome 4.3
Uniprot IDP17108
Protein Accession #NP_446288.1
Nucleotide Accession #NM_053836.1
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