IGF1 ELISA Kit (Sheep) (OKCA01020)

Catalog No: OKCA01020

Size:96 Wells

Sheep IGF1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Sheep
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	86	80-95
  1:2	95	87-101
  1:4	97	92-103
  1:8	93	82-98

• ELISA Kit Principle: Aviva Systems Biology IGF1 ELISA Kit (Sheep) (OKCA01020) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for IGF1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGF1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IGF1 captured in well.
• ELISA Kit Range: 15.6 ng/mL-1,000 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	90	87-92
  EDTA Plasma (n=4)	97	90-102

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level IGF1 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level IGF1 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • IGF1 Microplate: 96 Wells (12 x 8 Well strips)
  • IGF1 Lyophilized Standard: 2
  • 100X Biotinylated IGF1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 3.9 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IGF1
• Gene Full Name: Insulin-like growth factor I
• Alias Symbols: IGF-1, IGF-I
• NCBI Gene Id: 443318
• Protein Name: Insulin-like growth factor I
• Description of Target: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.
• Uniprot ID: P10763
• Protein Accession #: NP_001009774.1
• Nucleotide Accession #: NM_001009774.3