IDH2 Antibody (OAEB00968)

Catalog No: OAEB00968 (Formerly GWB-EV1048)

Size:100UG

OAEB00968 (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Epitope Info: Internal region
  Immunogen: Peptide with sequence CIHGLSNVKLNE, from the internal region of the protein sequence according to NP_002159.2.
  Reactivity Info: Tested: Human, Mouse, Rat; Expected: Human, Mouse, Rat, Dog, Cow, Pig
• Reconstitution and Storage: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
  Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: IDH2
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Human: 100%; Pig: 100%; Rabbit: 100%; Zebrafish: 83%
• Peptide Sequence: CIHGLSNVKLNE
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:16000.
  Western Blot: Approx 48kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 50.9kDa according to NP_002159.2). Recommended concentration: 0.1-0.3ug/ml.
• Reference: Abbas S, Lugthart S, Kavelaars FG, Schelen A, Koenders J, Zeilemaker A, van Putten WJ, Rijneveld A, Lowenberg B, Valk PJ.; Acquired mutations in the genes encoding IDH1 and IDH2 both are recurrent aberrations in acute myeloid leukemia (AML): prevalence and prognostic value.; Blood. 2010 Jun 10.; PMID: 20538800

Target Info

• Gene Symbol: Idh2|IDH2
• Gene Full Name: isocitrate dehydrogenase (NADP(+)) 2|isocitrate dehydrogenase 2 (NADP+), mitochondrial
• Alias Symbols: D2HGA2;E430004F23;ICD-M;ID;IDH;Idh-;Idh-2;IDHM;IDP;IDPM;isocitrate dehydrogenase (NADP(+)) 2, mitochondrial;isocitrate dehydrogenase [NADP], mitochondrial;isocitrate dehydrogenase 2 (NADP+), mitochondrial;mNADP-IDH;NADP(+)-specific ICDH;NADP+-specific isocitrate dehydrogenase;oxalosuccinate decarboxylase.
• NCBI Gene Id: 269951|3418|361596
• Protein Name: Isocitrate dehydrogenase [NADP], mitochondrial
• Uniprot ID: P48735
• Protein Accession #: NP_002159.2
• Molecular Weight: 50.9