HSD3B1 ELISA Kit (Rat) : 96 Wells (OKEH01671)

Catalog No: OKEH01671

Size:96T

Rat HSD3B1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology HSD3B1 ELISA Kit (Rat) (OKEH01671) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSD3B1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSD3B1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSD3B1 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.6% (n = 20)
  Mean Inter-assay CV%: < 8.2% (n = 20)
• ELISA Kit Component:
  • HSD3B1 Microplate: 96 Wells (12 x 8 Well strips)
  • HSD3B1 Lyophilized Standard: 2
  • 100X Biotinylated HSD3B1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 39.2 pg/mL
• Specificity: Natural and recombinant Rat 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Hsd3b1
• Gene Full Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
• Alias Symbols: 3 beta-hydroxysteroid dehydrogenase 1;3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1;3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I;3betaHSD;3-beta-HSD I;3-beta-hydroxy-5-ene steroid dehydrogenase;3-beta-hydroxy-Delta(5)-steroid dehydrogenase;3-beta-hydroxysteroid 3-dehydrogenase;delta-5-3-ketosteroid isomerase;dihydrotestosterone oxidoreductase;hydroxysteroid dehydrogenase-1, delta<5>-3-beta;steroid Delta-isomerase.
• NCBI Gene Id: 360348
• Protein Name: P22071
• Description of Target: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
• Uniprot ID: https://www.uniprot.org/uniprot/P22071
• Protein Accession #: NP_001007720.3
• Nucleotide Accession #: NM_001007719.3
• Protein Size (# AA): 373
• Molecular Weight: 42 kDa