HSD17B6 Polyclonal Antibody (OABB00344)

Catalog No: OABB00344

Size:100UG

Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Liver Tissue Lysate

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Bovine|Human
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Hydroxysteroid 17-beta dehydrogenase 6 is an enzyme that in humans is encoded by the HSD17B6 gene. The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. Baker ME et al point out expression of 17beta-HSDs had an important role in the early evolution of the physiological response to androgens and estrogens. Biswas and Russell concluded that 17beta-HSD6 and RoDH play opposing roles in androgen action; 17beta-HSD6 inactivates 3alpha-adiol by conversion to androsterone and RoDH activates 3alpha-adiol by conversion to dihydrotestosterone. The synthesis of an active steroid hormone by back conversion of an inactive metabolite represents a potentially important mechanism by which the steady state level of a transcriptional effector can be regulated.
  Background: Hydroxysteroid 17-beta dehydrogenase 6 is an enzyme that in humans is encoded by the HSD17B6 gene. The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. Baker ME et al point out expression of 17beta-HSDs had an important role in the early evolution of the physiological response to androgens and estrogens. Biswas and Russell concluded that 17beta-HSD6 and RoDH play opposing roles in androgen action; 17beta-HSD6 inactivates 3alpha-adiol by conversion to androsterone and RoDH activates 3alpha-adiol by conversion to dihydrotestosterone. The synthesis of an active steroid hormone by back conversion of an inactive metabolite represents a potentially important mechanism by which the steady state level of a transcriptional effector can be regulated.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
• Reference: 1. Baker ME (Feb 2001). "Evolution of 17beta-hydroxysteroid dehydrogenases and their role in
  androgen, estrogen and retinoid action". Mol Cell Endocrinol 171 (1-2): 211–5.
  doi:10.1016/S0303-7207(00)00414-7. PMID 11165032.
  2. Biswas, M. G., Russell, D. W. Expression cloning and characterization of oxidative 17-beta- and
  3-alpha-hydroxysteroid dehydrogenases from rat and human prostate. J. Biol. Chem. 272:
  15959-15966, 1997
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for 17-beta-hydroxysteroid dehydrogenase type 6(HSD17B6) detection. Tested with WB, IHC-P in Human.

Target Info

• Gene Symbol: HSD17B6
• Gene Full Name: hydroxysteroid 17-beta dehydrogenase 6
• Alias Symbols: 17-beta-HSD 6;17-beta-hydroxysteroid dehydrogenase type 6;17-beta-hydroxysteroid dehydrogenase type 6 variant 1;17-beta-hydroxysteroid dehydrogenase type 6 variant 2;17-beta-hydroxysteroid dehydrogenase type 6 variant 3;3(alpha->beta)-hydroxysteroid epimerase;3(alpha->beta)-hydroxysteroid epimerasel;3-alpha->beta-HSE;3-alpha->beta-hydroxysteroid epimerase;3-hydroxysteroid epimerase;HSE;hydroxysteroid (17-beta) dehydrogenase 6 homolog;NAD+ -dependent 3 alpha-hydroxysteroid dehydrogenase 3-hydroxysteroid epimerase;Oxidative 3-alpha hydroxysteroid dehydrogenase;oxidative 3-alpha-hydroxysteroid-dehydrogenase;oxidoreductase;retinol dehydrogenase;RODH;SDR9C6;short chain dehydrogenase/reductase family 9C member 6.
• NCBI Gene Id: 8630
• Protein Name: 17-beta-hydroxysteroid dehydrogenase type 6
• Description of Target: NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha-androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids.
• Uniprot ID: O14756
• Molecular Weight: 35966 MW