HNRNPA1 Antibody (OAGA02683)

Catalog No: OAGA02683

Size:100 ul

hnRNP A1 antibody detects HNRNPA1 protein by Western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
12 % SDS-PAGE
hnRNP A1 antibody (OAGA02683) dilution: 1:1000

Sample (30 ug of whole cell lysate)
A:H1299
B:HeLa S3
12% SDS PAGE
OAGA02683 diluted at 1:500

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
OAGA02683 diluted at 1:1000

hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
hnRNP A1 antibody (OAGA02683) diluted at 1:500.

hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
hnRNP A1 antibody (OAGA02683) diluted at 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using hnRNP A1 (OAGA02683) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human hnRNP A1. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.46mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: HNRNPA1
• Gene Full Name: heterogeneous nuclear ribonucleoprotein A1
• Alias Symbols: UP 1, ALS19, ALS20, HNRPA1, IBMPFD3, HNRPA1L3, hnRNP A1, hnRNP-A1
• NCBI Gene Id: 3178
• Description of Target: This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites.
• Uniprot ID: P09651