GSTM3 ELISA Kit (Human) (OKCD01110)

Catalog No: OKCD01110 (Formerly GWB-KBBCT8)

Size:96WELLS

Human GSTM3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	81-103%	82-102%	78-98%	87-99%
  EDTA plasma(n=5)	89-101%	93-105%	97-104%	82-96%
  heparin plasma(n=5)	92-104%	92-105%	82-96%	96-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Mu 3, Brain (GSTm3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Mu 3, Brain (GSTm3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Mu 3, Brain (GSTm3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutathione S Transferase Mu 3, Brain (GSTm3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	82-102	95
  EDTA plasma(n=5)	87-98	94
  heparin plasma(n=5)	97-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase Mu 3, Brain (GSTm3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Mu 3, Brain (GSTm3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GSTM3 Microplate: 96 Wells (12 x 8 Well Strips)
  • GSTM3 Lyophilized Standard: 2 x 200 ng
  • 100X Biotinylated GSTM3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 1.15 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Mu 3, Brain (GSTm3). No significant cross-reactivity or interference between Glutathione S Transferase Mu 3, Brain (GSTm3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GSTM3
• Gene Full Name: glutathione S-transferase mu 3
• Alias Symbols: brain GST;brain type mu-glutathione S-transferase;epididymis secretory sperm binding protein;glutathione S-alkyltransferase M3;glutathione S-aralkyltransferase M3;glutathione S-aryltransferase M3;glutathione S-transferase M3 (brain);glutathione S-transferase Mu 3;glutathione S-transferase mu 3 (brain);glutathione S-transferase, Mu-3;GST class-mu 3;GST5;GSTB;GSTM3-3;GTM3;hGSTM3-3;S-(hydroxyalkyl)glutathione lyase M3.
• NCBI Gene Id: 2947
• Protein Name: Glutathione S-transferase Mu 3
• Description of Target: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.
• Uniprot ID: P21266
• Protein Accession #: NP_000840
• Nucleotide Accession #: NM_000849.4
• Protein Size (# AA): 225