Goat Anti-SSA1 / Ro52 Antibody (OAEB00001)

Catalog No: OAEB00001 (Formerly GWB-7E9100)

Size:100UG

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with OAEB00001 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with OAEB00001 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Epitope Info: C Terminus
  Domains: B-box zinc finger; Domain in SPla and the RYanodine Receptor; Zinc finger, C3HC4 type (RING finger); associated with SPRY domains
  Reactivity Info: Tested: Human; Expected: Human
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: TRIM21
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human: 100%
• Peptide Sequence: CPLNIGSQGSTDY
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:16000.
  Western Blot: In transfected HEK293 transiently expressing full-length Human TRIM21 (myc and DYKDDDDK tagged), a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1ug/ml.
• Reference: Chan EK, Di Donato F, Hamel JC, Tseng CE, Buyon JP.
  52-kD SS-A/Ro: genomic structure and identification of an alternatively spliced transcript encoding a novel leucine zipper-minus autoantigen expressed in fetal and adult heart.
  J Exp Med. 1995 Oct 1;182(4):983-92. PMID: 7561701

Target Info

• Gene Symbol: TRIM21
• Gene Full Name: tripartite motif containing 21
• Alias Symbols: 52 kDa ribonucleoprotein autoantigen Ro/SS-A;52 kDa Ro protein;E3 ubiquitin-protein ligase TRIM21;RING finger protein 81;RING-type E3 ubiquitin transferase TRIM21;RNF81;ro(SS-A);Ro/SSA;Ro/SSA 52kDa;RO52;Sicca syndrome antigen A;sjoegren syndrome type A antigen;Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro);SSA;SS-A;SSA1;tripartite motif-containing protein 21.
• NCBI Gene Id: 6737
• Protein Name: E3 ubiquitin-protein ligase TRIM21
• Uniprot ID: P19474
• Protein Accession #: NP_003132.2
• Molecular Weight: 54.2