Goat Anti-SPFH2 / ERLIN2 Antibody (OAEB01512)

Catalog No: OAEB01512 (Formerly GWB-661A6E)

Size:100ug

OAEB01512 staining (1ug/ml) of Human Breast lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Info

• Predicted Species Reactivity: Human, Rabbit
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: This antibody is expected to recognise isoform 1 (NP_009106) only.
• Predicted Homology Based on Immunogen Sequence: Human: 100%; Rabbit: 100%
• Peptide Sequence: C-EDEPLETATKEN
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:2000.
  Western Blot: Approx 43kDa band observed in Human Breast lysates and in lysates of Human Breast cancer (calculated MW of 37.8kDa according to NP_009106.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pearce et al, J Biol Chem. 2007 Jul 13;282(28):20104-15.; PMID: 17502376). Recommended concentration: 0.3-1ug/ml.
• Reference: * Garcia MJ, Pole JC, Chin SF, Teschendorff A, Naderi A, Ozdag H, Vias M, Kranjac T, Subkhankulova T, Paish C, Ellis I, Brenton JD, Edwards PA, Caldas C.; A 1 Mb minimal amplicon at 8p11-12 in breast cancer identifies new candidate oncogenes.; Oncogene. 2005 Aug 4;24(33):5235-45.; Pubmed 15897872

Target Info

• Gene Symbol: ERLIN2
• Alias Symbols: NET32, SPFH2, SPG18, C8orf2, Erlin-2
• NCBI Gene Id: 11160
• Protein Name: Erlin-2
• Uniprot ID: O94905
• Protein Accession #: NP_009106.1