Goat Anti-BIF-1 / SH3GLB1 Antibody (OAEB01689)

Catalog No: OAEB01689 (Formerly GWB-BCD966)

Size:100UG

OAEB01689 staining (0.3ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: SH3GLB1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 92%; Horse: 92%; Human: 100%; Pig: 92%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Peptide Sequence: C-QKGKVPITYLELLN
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:32000.
  Western Blot: Approx 38kDa band observed in lysates of Human T cell line Jurkat and in Human Lung and Placenta (calculated MW of 40.8kDa according to NP_057093.1). Recommended concentration: 0.3-1.0 ug/ml. A minor band of unknown identity was also consistently observed at 50kDa in Jurkat and Placenta. This band was successfully blocked by incubation with the immunising peptide.
• Reference: * Takahashi Y, Karbowski M, Yamaguchi H, Kazi A, Wu J, Sebti SM, Youle RJ, Wang HG.; Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis.; Mol Cell Biol. 2005 Nov;25(21):9369-82.; Pubmed 16227588
• Publications:

  NKCT1 (purified Naja kaouthia protein toxin) conjugated gold nanoparticles induced Akt/mTOR inactivation mediated autophagic and caspase 3 activated apoptotic cell death in leukemic cell. Toxicon. 121, 86-97 (2016). 27527270

Target Info

• Gene Symbol: Sh3glb1|SH3GLB1
• Gene Full Name: SH3 domain containing GRB2 like, endophilin B1|SH3-domain GRB2-like B1 (endophilin)
• Alias Symbols: AA409932;AI314629;AU015566;Bax-interacting factor 1;Bif-;Bif-1;CGI-61;dJ612B15.2;endophilin-B1;mKIAA0491;PPP1R70;protein phosphatase 1, regulatory subunit 70;SH3 domain-containing GRB2-like protein B1;SH3-containing protein SH3GLB1;SH3-domain GRB2 like endophilin B1;testicular tissue protein Li 172.
• NCBI Gene Id: 51100|54673
• Protein Name: Endophilin-B1
• Uniprot ID: Q9Y371
• Protein Accession #: NP_057093.1
• Molecular Weight: 40.8; 44.3; 43.3; 29.3