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Goat Anti-BIF-1 / SH3GLB1 Antibody (OAEB01689)

100 ug
$349.00
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Gene Symbol:
SH3GLB1
NCBI Gene Id:
51100
Protein Name:
Endophilin-B1
Swissprot Id:
Q9Y371
Protein Accession #:
NP_057093.1
Alias Symbols:
Bif-1, endophilin B1, SH3-domain GRB2-like endophilin B1, SH3GLB1
Replacement Item:
This antibody may replace item sc-120038 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express SH3GLB1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express SH3GLB1.
Predicted Homology Based on Immunogen Sequence:
Dog: 92%; Horse: 92%; Human: 100%; Pig: 92%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
Complete computational species homology data:
SH3GLB1 Antibody (OAEB01689)
Peptide Sequence:
C-QKGKVPITYLELLN
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-SH3GLB1 antibody - OAEB01689
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: Approx 38kDa band observed in lysates of Human T cell line Jurkat and in Human Lung and Placenta (calculated MW of 40.8kDa according to NP_057093.1). Recommended concentration: 0.3-1.0 ug/ml. A minor band of unknown identity was also consistently observed at 50kDa in Jurkat and Placenta. This band was successfully blocked by incubation with the immunising peptide.
Target Reference:
* Takahashi Y, Karbowski M, Yamaguchi H, Kazi A, Wu J, Sebti SM, Youle RJ, Wang HG.; Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis.; Mol Cell Biol. 2005 Nov;25(21):9369-82.; Pubmed 16227588
Publications:

Bhowmik, T; Gomes, A; NKCT1 (purified Naja kaouthia protein toxin) conjugated gold nanoparticles induced Akt/mTOR inactivation mediated autophagic and caspase 3 activated apoptotic cell death in leukemic cell. 121, 86-97 (2016). WB, Dog, Horse, Human, Pig, Rabbit, Rat, Zebrafish 27527270

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