| Product Number |
OAEB01689 |
| Product Page |
www.avivasysbio.com/goat-anti-bif-1-sh3glb1-antibody-oaeb01689.html |
| Name |
Goat Anti-BIF-1 / SH3GLB1 Antibody (OAEB01689) |
| Molecular Weight |
40.8; 44.3; 43.3; 29.3 |
| NCBI Gene Id |
51100|54673 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
SH3 domain containing GRB2 like, endophilin B1|SH3-domain GRB2-like B1 (endophilin) |
| Alias Symbols |
AA409932;AI314629;AU015566;Bax-interacting factor 1;Bif-;Bif-1;CGI-61;dJ612B15.2;endophilin-B1;mKIAA0491;PPP1R70;protein phosphatase 1, regulatory subunit 70;SH3 domain-containing GRB2-like protein B1;SH3-containing protein SH3GLB1;SH3-domain GRB2 like endophilin B1;testicular tissue protein Li 172. |
| Peptide Sequence |
C-QKGKVPITYLELLN |
| Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reference |
* Takahashi Y, Karbowski M, Yamaguchi H, Kazi A, Wu J, Sebti SM, Youle RJ, Wang HG.; Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis.; Mol Cell Biol. 2005 Nov;25(21):9369-82.; Pubmed 16227588 |
| Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for Goat Anti-BIF-1 / SH3GLB1 Antibody (OAEB01689) |
| Application Info |
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: Approx 38kDa band observed in lysates of Human T cell line Jurkat and in Human Lung and Placenta (calculated MW of 40.8kDa according to NP_057093.1). Recommended concentration: 0.3-1.0 ug/ml. A minor band of unknown identity was also consistently observed at 50kDa in Jurkat and Placenta. This band was successfully blocked by incubation with the immunising peptide. |
| Immunogen |
SH3GLB1 |
| Uniprot ID |
Q9Y371 |
| Protein Name |
Endophilin-B1 |
| Publications |
NKCT1 (purified Naja kaouthia protein toxin) conjugated gold nanoparticles induced Akt/mTOR inactivation mediated autophagic and caspase 3 activated apoptotic cell death in leukemic cell. Toxicon. 121, 86-97 (2016). 27527270 |
| Protein Accession # |
NP_057093.1 |
| Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
| Gene Symbol |
Sh3glb1|SH3GLB1 |
| Predicted Species Reactivity |
Bovine|Dog|Human|Mouse|Rat |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Dog: 92%; Horse: 92%; Human: 100%; Pig: 92%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
| Image 1 | Jurkat
 | OAEB01689 staining (0.3ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
|
