Goat Anti-ANILLIN / Scraps (C Terminus) Antibody (OAEB00916)

Catalog No: OAEB00916 (Formerly GWB-F8C8B9)

Size:100UG

OAEB00916 (10ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: ANLN
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 92%; Pig: 100%; Rabbit: 100%
• Peptide Sequence: WQPDACYKPIGKP
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:64000.
  Western Blot: Preliminary experiments gave an approx 75kDa band in Mouse Brain lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124kDa according to NP_061155.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
• Reference: * Hall PA, Todd CB, Hyland PL, McDade SS, Grabsch H, Dattani M, Hillan KJ, Russell SE.; The septin-binding protein anillin is overexpressed in diverse human tumors.; Clin Cancer Res. 2005 Oct 1;11(19 Pt 1):6780-6.; Pubmed 16203764
  * Oegema K, Savoian MS, Mitchison TJ, Field CM.; Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis.; J Cell Biol. 2000 Aug 7;150(3):539-52.; Pubmed 10931866

Target Info

• Gene Symbol: Anln|ANLN
• Gene Full Name: anillin actin binding protein|anillin, actin binding protein
• Alias Symbols: 1110037A17Rik;2900037I21Rik;actin-binding protein anillin;anillin;anillin, actin binding protein (scraps homolog, Drosophila);C78101;FSGS8;S;scra;Scraps.
• NCBI Gene Id: 54443|68743
• Protein Name: Actin-binding protein anillin
• Uniprot ID: Q9NQW6
• Protein Accession #: NP_061155.2
• Molecular Weight: 124; 120; 120