Goat Anti-ANILLIN / Scraps (C Terminus) Antibody (OAEB00916)

Data Sheet
 
Product Number OAEB00916
Product Page www.avivasysbio.com/goat-anti-anillin-scraps-c-terminus-antibody-oaeb00916.html
Name Goat Anti-ANILLIN / Scraps (C Terminus) Antibody (OAEB00916)
Molecular Weight 124; 120; 120
NCBI Gene Id 54443|68743
Host Goat
Clonality Polyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration 0.5 mg/ml
Gene Full Name anillin actin binding protein|anillin, actin binding protein
Alias Symbols 1110037A17Rik;2900037I21Rik;actin-binding protein anillin;anillin;anillin, actin binding protein (scraps homolog, Drosophila);C78101;FSGS8;S;scra;Scraps.
Peptide Sequence WQPDACYKPIGKP
Product Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reference * Hall PA, Todd CB, Hyland PL, McDade SS, Grabsch H, Dattani M, Hillan KJ, Russell SE.; The septin-binding protein anillin is overexpressed in diverse human tumors.; Clin Cancer Res. 2005 Oct 1;11(19 Pt 1):6780-6.; Pubmed 16203764
* Oegema K, Savoian MS, Mitchison TJ, Field CM.; Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis.; J Cell Biol. 2000 Aug 7;150(3):539-52.; Pubmed 10931866
Reconstitution and Storage Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals Printable datasheet for Goat Anti-ANILLIN / Scraps (C Terminus) Antibody (OAEB00916)
Application Info Peptide ELISA: antibody detection limit dilution 1:64000.
Western Blot: Preliminary experiments gave an approx 75kDa band in Mouse Brain lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124kDa according to NP_061155.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen ANLN
Uniprot ID Q9NQW6
Protein Name Actin-binding protein anillin
Protein Accession # NP_061155.2
Purification Ammonium Sulfate Precipitated; Affinity Purified
Gene Symbol Anln|ANLN
Predicted Species Reactivity Bovine|Human|Mouse
Application IHC
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 92%; Pig: 100%; Rabbit: 100%
Image 1
Human Kidney
OAEB00916 (10ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
 

AVIVA SYSTEMS BIOLOGY manufactures and sells quality antibody products covering genome wide proteins.

AVIVA SYSTEMS BIOLOGY
10211 Pacific Mesa Blvd, Ste 401, San Diego, CA 92121 USA | Tel: (858)552-6979 | info@avivasysbio.com