Goat Anti-ADAM17 / TACE Antibody (OAEB01134)

Catalog No: OAEB01134 (Formerly GWB-2B32DE)

Size:100UG

OAEB01134 staining (0.2ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC, P-ELISA
• Reconstitution and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: ADAM17
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Peptide Sequence: LQRQNRVDSKETEC
• Concentration: 0.5 mg/ml
• Specificity: This antibody is expected to recognise isoform 1 (represented by NP_003174.3) and not isoform 2 (represented by NP_068604).
• Application Info: P-ELISA: Antibody detection limit dilution 1:128000
  WB: Approx 100kDa band observed in Human Testis lysates and approx 125kDa in lysates of cell lines Daudi, HeLa and Jurkat (calculated MW of 93.0kDa according to NP_003174.3). The observed molecular weights correspond to earlier findings in literature with different antibodies (Le Gall et al, J Cell Sci. 2010 Nov 15;123(Pt22):3913-22; PMID: 20980382). Recommended concentration: 0.1-0.3 ug/ml. Primary incubated for 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.
  IHC: Paraffin embedded Human Skin and Thyroid. Recommended concentration: 3.75 ug/ml.
• Reference: * Black RA, Rauch CT, Kozlosky CJ, Peschon JJ, Slack JL, Wolfson MF, Castner BJ, Stocking KL, Reddy P, Srinivasan S, Nelson N, Boiani N, Schooley KA, Gerhart M, Davis R, Fitzner JN, Johnson RS, Paxton RJ, March CJ, Cerretti DP; A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells.; Nature. 1997 Feb 20;385(6618):729-33; Pubmed 9034190

Target Info

• Gene Symbol: Adam17|ADAM17
• Gene Full Name: a disintegrin and metallopeptidase domain 17|ADAM metallopeptidase domain 17
• Alias Symbols: a disintegrin and metalloproteinase 17;ADAM metallopeptidase domain 18;ADAM18;cartilage snake venom-like protease;CD156b;CSVP;disintegrin and metalloproteinase domain-containing protein 17;NISBD;NISBD1;snake venom-like protease;Tac;Tace;TNF-alpha convertase;TNF-alpha convertase enzyme;TNF-alpha converting enzyme;tumor necrosis factor, alpha, converting enzyme.
• NCBI Gene Id: 11491|6868
• Protein Name: Disintegrin and metalloproteinase domain-containing protein 17
• Uniprot ID: P78536
• Protein Accession #: NP_003174.3
• Molecular Weight: 93