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ADAM17 ELISA Kit (Human) : 96 Wells (OKEH00945)

96 Wells
$529.00
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Predicted Species Reactivity:
Human
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology ADAM17 ELISA Kit (Human) (OKEH00945) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAM17 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAM17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAM17 captured in well.
Kit Range:
0.312 - 20ng/mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
Kit Reproducibility:
Mean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 10.3% (n = 20)
Kit Component:
ComponentAmount
ADAM17 Microplate96 Wells (12 x 8 Well strips)
ADAM17 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ADAM17 Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
ADAM17
Official Gene Full Name:
ADAM metallopeptidase domain 17
Alias Symbols:
ADAM 17, ADAM18, CD156B, cSVP, CSVP, Disintegrin and metalloproteinase domain-containing protein 17, MGC71942, Snake venom-like protease, TACE, TNF-alpha convertase, TNF-alpha-converting enzyme
NCBI Gene Id:
6868
Protein Name:
Disintegrin and metalloproteinase domain-containing protein 17
Description of Target:
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. The encoded protease functions in the ectodomain shedding of tumor necrosis factor-alpha, in which soluble tumor necrosis factor-alpha is released from the membrane-bound precursor. This protease also functions in the processing of numerous other substrates, including cell adhesion proteins, cytokine and growth factor receptors and epidermal growth factor (EGF) receptor ligands. The encoded protein also plays a prominent role in the activation of the Notch signaling pathway. Elevated expression of this gene has been observed in specific cell types derived from psoriasis, rheumatoid arthritis, multiple sclerosis and Crohn's disease patients, suggesting that the encoded protein may play a role in autoimmune disease. [provided by RefSeq, Feb 2016]
Swissprot Id:
P78536
Protein Accession #:
NP_003174.3
Nucleotide Accession #:
NM_003183.5
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
0.11 ng/mL
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ADAM17.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ADAM17.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Human Disintegrin and metalloproteinase domain-containing protein 17
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Molecular Weight:
93 kDa
Protein Size (# AA):
824

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