GDF2 Chemi-Luminescent ELISA Kit (Human) (OKCD03517)

Catalog No: OKCD03517

Size:96WELLS

Human GDF2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	95-103%	97-105%	83-92%	90-97%
  EDTA Plasma (n=5)	89-104%	88-95%	79-89%	91-99%
  Heparin Plasma (n=5)	80-103%	94-102%	86-103%	80-91%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 9 (BMP9). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 9 (BMP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bone Morphogenetic Protein 9 (BMP9) level in the sample or standard.
• ELISA Kit Range: 68.6-50,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94-103	98
  EDTA Plasma (n=5)	78-101	94
  Heparin Plasma (n=5)	79-99	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 9 (BMP9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 9 (BMP9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • GDF2 Microplate: 96 Wells (12 x 8 Well strips)
  • GDF2 Lyophilized Standard: 2
  • 100X GDF2 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.78 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GDF2
• Gene Full Name: growth differentiation factor 2
• Alias Symbols: BMP9;BMP-9;bone morphogenetic protein 9;growth/differentiation factor 2;HHT5.
• NCBI Gene Id: 2658
• Protein Name: Growth/differentiation factor 2
• Description of Target: Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.
• Uniprot ID: Q9UK05
• Protein Accession #: NP_057288
• Nucleotide Accession #: NM_016204