Catalog No: OKEH04693
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology GDF2 ELISA Kit (Human) (OKEH04693) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GDF2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GDF2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GDF2 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
GDF2 Microplate96 Wells (12 x 8 Well Strips)
GDF2 Standard2
Sample Diluent1 x 20 mL
GDF2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.34 ng/mL
SpecificityNatural and recombinant Human Growth/differentiation factor 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGDF2
Gene Full Namegrowth differentiation factor 2
Alias SymbolsBMP9;BMP-9;bone morphogenetic protein 9;growth/differentiation factor 2;HHT5.
NCBI Gene Id2658
Protein NameQ9UK05
Description of TargetGrowth/differentiation factor 2
Uniprot ID
Protein Accession #NP_057288.1
Nucleotide Accession #NM_016204.2
Protein Size (# AA)429
Molecular Weight47 kDa
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