GCDH ELISA Kit (Mouse) (OKEH06494)

Catalog No: OKEH06494

Size:96T

Mouse GCDH ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Gcdh ELISA Kit (Mouse) (OKEH06494) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Gcdh antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Gcdh and incubated. The Gcdh found in the sample or standards competes with the biotinylated Gcdh for limited binding sites on the immobilized anti-Gcdh antibody. Excess unbound biotinylated Gcdh and sample or standard Gcdh is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Gcdh captured in the well and inversely proportional to the amount of Gcdh which was contained in the sample or standard.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 88.8%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <= 5.4% (n = 20)
  Mean Inter-Assay Precision - <= 8.0% (n = 20)
• ELISA Kit Component:
  • H3F3C Microplate: 96 Wells (12 x 8 Well strips)
  • H3F3C Lyophilized Standard: 2
  • 100X Biotinylated H3F3C Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.321 ng/mL
• Specificity: Natural and recombinant Mouse Glutaryl-CoA dehydrogenase, mitochondrial
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Gcdh
• Gene Full Name: glutaryl-Coenzyme A dehydrogenase
• Alias Symbols: 9030411L18;AI266902;D17825;GCD;glutaryl-CoA dehydrogenase, mitochondrial.
• NCBI Gene Id: 270076
• Protein Name: Q60759
• Description of Target: Glutaryl-CoA dehydrogenase, mitochondrial
• Uniprot ID: https://www.uniprot.org/uniprot/Q60759
• Protein Accession #: NP_058599
• Nucleotide Accession #: NM_016903