FLII ELISA Kit (Human) (OKCD01219)

Catalog No: OKCD01219 (Formerly GWB-KBBPP6)

Size:96WELLS

Human FLII ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Flightless I Homolog (FLII). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Flightless I Homolog (FLII). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Flightless I Homolog (FLII), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Flightless I Homolog (FLII) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Flightless I Homolog (FLII) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Flightless I Homolog (FLII) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-FLII Microplate: 96 Wells (12 x 8 Well Strips)
  • FLII Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated FLII Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 29.2 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Flightless I Homolog (FLII). No significant cross-reactivity or interference between Flightless I Homolog (FLII) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: FLII
• Gene Full Name: FLII actin remodeling protein
• Alias Symbols: FLI;Fli1;flightless I actin binding protein;flightless I homolog;FLIL;protein flightless-1 homolog.
• NCBI Gene Id: 2314
• Protein Name: Protein flightless-1 homolog
• Description of Target: May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.
• Uniprot ID: Q13045
• Protein Accession #: NP_001243193
• Nucleotide Accession #: NM_001256264.1
• Protein Size (# AA): 1269