FGF2 Antibody - middle region (OAAB00137)

Catalog No: OAAB00137

Size:400ul

FGF2 Antibody (Center) western blot analysis in NCI-H460cell lysates (35ug/lane).This demonstrates the FGF2 antibody detected the FGF2 protein (arrow).

FGF2 is strongly supported by BioGPS gene expression data to be expressed in NCI-H460

FGF2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FGF2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P, IF
• Additional Information: Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
  Cellular Location: Secreted. Nucleus. Note=Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  Tissue Location: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This FGF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-191 amino acids from the Central region of human FGF2.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Cow; Chicken; Mouse; Rabbit; Rat
• Sample Type Confirmation:

  FGF2 is strongly supported by BioGPS gene expression data to be expressed in NCI-H460

• Application Info: WB: 1:2000
  IHC-P: 1:10~50
  IF: 1:10~50

Target Info

• Gene Symbol: FGF2
• Alias Symbols: BFGF, FGFB, FGF-2, HBGF-2
• NCBI Gene Id: 2247
• Protein Name: Fibroblast growth factor 2
• Description of Target: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
  
• Uniprot ID: P09038
• Protein Accession #: NP_001997.5
• Molecular Weight: 30770 Da