FCGRT ELISA Kit (Rat) (OKCA02561)

Catalog No: OKCA02561

Size:96 Wells

Rat FCGRT ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	86	82-91
  1:2	102	97-107
  1:4	91	85-97
  1:8	95	92-101

• ELISA Kit Principle: Aviva Systems Biology Fcgrt ELISA Kit (Rat) (OKCA02561) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fcgrt has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fcgrt is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Fcgrt captured in the well.
• ELISA Kit Range: 25 - 1,600 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	93	88-96
  EDTA Plasma (n=4)	98	94-102

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • Fcgrt Microplate: 96 Wells (12 x 8 Well strips)
  • Fcgrt Lyophilized Standard: 2
  • 100X Biotinylated Fcgrt Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 6.25 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: FCGRT
• Alias Symbols: FcRn
• NCBI Gene Id: 29558
• Protein Name: IgG receptor FcRn large subunit p51
• Description of Target: Binds to the Fc region of monomeric immunoglobulins gamma . Mediates the selective uptake of IgG from milk and helps newborn animals to acquire passive immunity. IgG in the milk is bound at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids . Possible role in transfer of immunoglobulin G from mother to fetus.
• Uniprot ID: P13599
• Protein Accession #: NP_203502
• Nucleotide Accession #: NM_033351