FBL Protein (OPPA02512)

Catalog No: OPPA02512

Size:1ug

Product Info

• Product Format: The FBL solution (0.5 mg/ml) contains 20mM Tris-HCl buffer(pH 8.0), 20% glycerol 1mM DTT, 0.2M NaCl, 1mM EDTA. Physical appearance: Sterile Filtered colorless solution.
• Host: E. Coli
  Product Introduction: FBL is a significant small nucleolar protein in eukaryotes, which has an essential role in pre-rRNA processing during ribosomal biogenesis. Fibrillarin is a component of several ribonucleoproteins including a nucleolar small nuclear ribonucleoprotein (SnRNP) and one of the two classes of small nucleolar ribonucleoproteins (snoRNPs). Fibrillarin contains an N-terminal repetitive domain which is rich in glycine and arginine residues, like fibrillarins in other species. Fibrillarin's central region is similar to an RNA-binding domain and contains an RNP consensus sequence. FBL is linked to the U3, U8, and U13 small nuclear RNAs and is positioned in the dense fibrillar component (DFC) of the nucleolus. Antisera from roughly 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.
  Product Description: FBL Human Recombinant fused with 23 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 262 amino acids (83-321 a.a.) and having a molecular mass of 28.9kDa. The FBL is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: FBL although stable 4C for 4 weeks, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 85.0% as determined by SDS-PAGE.
• Peptide Sequence: MGSSHHHHHH SSGLVPRGSH RSMGKNVMVE PHRHEGVFIC RGKEDALVTK NLVPGESVYG EKRVSISEGD DKIEYRAWNP FRSKLAAAIL GGVDQIHIKP GAKVLYLGAA SGTTVSHVSD IVGPDGLVYA VEFSHRSGRD LINLAKKRTN IIPVIEDARH PHKYRMLIAM VDVIFADVAQ PDQTRIVALN AHTFLRNGGH FVISIKANCI DSTASAEAVF ASEVKKMQQE NMKPQEQLTL EPYERDHAVV VGVYRPPPKV KN.

Target Info

• Gene Symbol: FBL
• Alias Symbols: FIB, FLRN, Nop1, RNU3IP1
• NCBI Gene Id: 2091
• Protein Name: rRNA 2'-O-methyltransferase fibrillarin
• Description of Target: Recombinant human Fibrillarin
• Uniprot ID: P22087
• Protein Accession #: NP_001427.2