FBL Antibody (OAGA04399)

Catalog No: OAGA04399

Size:100 ul

Fibrillarin antibody detects FBL protein by Western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
12 % SDS-PAGE
Fibrillarin antibody dilution: 1:500

Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 ug) and nuclear extract(30 ug) from A549 cells
10 % SDS-PAGE
Fibrillarin antibody dilution: 1:500

Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 ug) and nuclear extract (30 ug) from Jurkat cells
10 % SDS-PAGE
Fibrillarin antibody dilution: 1:500

Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 ug) and nuclear extract (30 ug) from SK-N-SH cells
10 % SDS-PAGE
Fibrillarin antibody dilution: 1:500

Fibrillarin antibody detects Fibrillarin protein by western blot analysis.
whole cell lysate (30 ug) and nuclear extract (30 ug) from IMR32 cells
10 % SDS-PAGE
Fibrillarin antibody dilution: 1:500

Fibrillarin antibody detects FBL protein by Western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
12 % SDS-PAGE
Fibrillarin antibody dilution: 1:1000

Sample (30 ug of whole cell lysate)
A: 293T
B: H1299
C: Molt-4
12% SDS PAGE
diluted at 1:1000

Fibrillarin antibody detects Fibrillarin protein at nucleolus in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
Fibrillarin antibody diluted at 1:500.

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using Fibrillarin antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibrillarin antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Fibrillarin. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.36 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: FBL
• Gene Full Name: fibrillarin
• Alias Symbols: FIB, FLRN, Nop1, RNU3IP1
• NCBI Gene Id: 2091
• Protein Name: rRNA 2'-O-methyltransferase fibrillarin
• Description of Target: This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.
• Uniprot ID: P22087
• Protein Accession #: NP_001427.2
• Nucleotide Accession #: NM_001436.3