FASN Chemi-Luminescent ELISA Kit (Chicken) (OKCD03754)

Catalog No: OKCD03754

Size:96WELLS

Gallus FASN ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Chicken|Gallus gallus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-95%	87-102%	86-99%	80-96%
  EDTA Plasma (n=5)	90-97%	84-91%	89-99%	92-101%
  Heparin Plasma (n=5)	98-105%	99-105%	79-102%	83-105%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Synthase (FASN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fatty Acid Synthase (FASN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fatty Acid Synthase (FASN) level in the sample or standard.
• ELISA Kit Range: 5.49-4,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	93-101	96
  EDTA Plasma (n=5)	86-103	89
  Heparin Plasma (n=5)	83-101	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Fasn Microplate: 96 Wells (12 x 8 Well strips)
  • Fasn Lyophilized Standard: 2
  • 100X Fasn Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 2.5 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Fasn
• Alias Symbols: FAS, Fatty acid synthase
• NCBI Gene Id: 396061
• Protein Name: Fatty acid synthase
• Description of Target: Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities as an acyl carrier protein.
• Uniprot ID: P12276
• Protein Accession #: NP_990486
• Nucleotide Accession #: NM_205155