Catalog No: OKEH04869
Size:96T
Price: $525.00
SKU
OKEH04869
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH04869
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Fasn ELISA Kit (Mouse) (OKEH04869) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fasn has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fasn is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fasn captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Fasn Microplate96 Wells (12 x 8 Well Strips)
Fasn Standard2
Sample Diluent1 x 20 mL
Fasn Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity7.98 pg/mL
SpecificityNatural and recombinant Mouse Fatty acid synthase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFasn
Gene Full Namefatty acid synthase
Alias SymbolsA630082H08Rik;F;FAS;fatty acid synthase;type I Fatty Acid Synthase.
NCBI Gene Id14104
Protein NameP19096
Description of TargetFatty acid synthase
Uniprot IDhttps://www.uniprot.org/uniprot/P19096
Protein Accession #NP_032014.3
Nucleotide Accession #NM_007988.3
Protein Size (# AA)2504
Molecular Weight272 kDa
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