FAS Antibody (OAEE00342)

Catalog No: OAEE00342

Size:0.1 mg

Induction of apoptosis in various hematopoetic cell lines by use of anti-Fas antibodies.
Suspensions of used cell lines were incubated with soluble (or without as control) anti-Fas antibodies. Induction of apoptosis was measured by Apoptosis Assay Kit - FITC.
Histograms:
Red (full) - untreated control cells
Black - standard apoptosis-inducing anti-Fas antibody
Green - anti-Fas (UT-1)

A - JURKAT human peripheral blood T cell leukemia cell line
B - TF-1 human bone marrow erythroleukemia cell line
C - CEM human leukemia cell line
D - MOLT-4 human acute lymphoblastic T cell leukemia cell line

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: UT-1
• Isotype: IgM
• Host: Mouse
• Application: FUNC, FC
• Reconstitution and Storage: Store at 2-8C. Do not freeze. Do not use after expiration date stamped on vial label.
• Immunogen: HUT-78 human T cell lymphoma cells
• Purification: Precipitation methods and ion exchange chromatography
• Concentration: 1 mg/ml
• Specificity: The antibody UT-1 reacts with CD95 (Fas/APO-1), a 46 kDa glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.
  The antibody UT-1 does excellently induce Fas mediated apoptosis, similarly as CH11 antibody.
• Formulation: Azide free
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 um filter sterilized.
• Reference: Matsuyama W, Mitsuyama H, Ono M, Shirahama Y, Higashimoto I, Osame M, Arimura K: Discoidin domain receptor 1 contributes to eosinophil survival in anNF-kappaB-dependent manner in Churg-Strauss syndrome. Blood. 2007 Jan 1;109(1):22-30.
  Brumatti G, Yon M, Castro FA, Bueno-da-Silva AE, Jacysyn JF, Brunner T, Amarante-Mendes GP: Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide. Exp Cell Res. 2008 Feb 1;314(3):554-63.
  Guo Z, Zhang M, Tang H, Cao X: Fas signal links innate and adaptive immunity by promoting dendritic-cellsecretion of CC and CXC chemokines. Blood. 2005 Sep 15;106(6):2033-41.
  Park DR, Thomsen AR, Frevert CW, Pham U, Skerrett SJ, Kiener PA, Liles WC: Fas (CD95) induces proinflammatory cytokine responses by human monocytes and monocyte-derived macrophages. J Immunol. 2003 Jun 15;170(12):6209-16.
  Scaffidi C, Fulda S, Srinivasan A, Friesen C, Li F, Tomaselli KJ, Debatin KM, Krammer PH, Peter ME: Two CD95 (APO-1/Fas) signaling pathways. EMBO J. 1998 Mar 16;17(6):1675-87.

Target Info

• Gene Symbol: FAS
• Gene Full Name: Tumor necrosis factor receptor superfamily member 6
• Alias Symbols: APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6
• NCBI Gene Id: 355
• Protein Name: Tumor necrosis factor receptor superfamily member 6
• Uniprot ID: P25445
• Protein Accession #: NP_000034.1; NP_690610.1; NP_690611.1
• Nucleotide Accession #: NM_000043.4; NM_152871.2; NM_152872.2