- Predicted Species Reactivity:
- Kit Detection Method:
- Colorimetric, OD450 nm
- Kit Duration:
- ~ 3 Hours
- Kit Principle:
- Aviva Systems Biology F10 ELISA Kit (Human) (OKEH04384) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F10 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for F10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample F10 captured in the well.
- Kit Range:
- 0.156 - 10ng/mL
- Kit Recovery:
- Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
- Kit Reproducibility:
- Mean Intra-assay CV%: <= 4.2% (n = 20)
Mean Inter-assay CV%: <= 8.1% (n = 20)
- Kit Component:
|Anti-F10 Microplate||96 Wells (12 x 8 Well Strips)|
|F10 Lyophilized Standard||2 x 2 ng|
|Sample Diluent||1 x 20 mL|
|F10 Biotinylated Detector Antibody||1 x 120 uL|
|Detector Antibody Diluent||1 x 12 mL|
|HRP Avidin Conjugate||1 x 120 uL|
|Conjugate Diluent||1 x 12 mL|
|25X Wash Buffer||1 x 30 mL|
|TMB Substrate||1 x 10 mL|
|Stop Solution||1 x 10 mL|
- Reconstitution and Storage:
- Store as indicated in product manual.
- Official Gene Full Name:
- coagulation factor X
- Alias Symbols:
- CXCLG16, C-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, SCYB16, Small-inducible cytokine B16, SRPSOX, SR-PSOX, Transmembrane chemokine CXCL16, UNQ2759/PRO6714
- NCBI Gene Id:
- Protein Name:
- Coagulation factor X
- Description of Target:
- This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.
- Sample Type:
- Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
- 0.098 ng/mL
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express F10.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express F10.
- Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
- Natural and recombinant Human Coagulation factor X