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F10 ELISA Kit (Human) (OKEH04384)

Catalog#: OKEH04384
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology F10 ELISA Kit (Human) (OKEH04384) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F10 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for F10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample F10 captured in the well.
Kit Range 0.156 - 10ng/mL
Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Kit Reproducibility Mean Intra-assay CV%: <= 4.2% (n = 20)
Mean Inter-assay CV%: <= 8.1% (n = 20)
Kit Component
Anti-F10 Microplate96 Wells (12 x 8 Well Strips)
F10 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
F10 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity 0.098 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Human Coagulation factor X
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol F10
Official Gene Full Name coagulation factor X
Alias Symbols CXCLG16, C-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, SCYB16, Small-inducible cytokine B16, SRPSOX, SR-PSOX, Transmembrane chemokine CXCL16, UNQ2759/PRO6714
NCBI Gene Id 2159
Protein Name Coagulation factor X
Description of Target This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.
Swissprot Id P00742
Protein Accession # NP_000495.1
Nucleotide Accession # NM_000504.3
Protein Size (# AA) 488
Molecular Weight 55 KDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express F10.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express F10.
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