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- $55: Antibody & Protein in US
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| Datasheets/Manuals | Printable datasheet for ENDOG Antibody (OAPB00172) (OAPB00172) |
|---|
| Predicted Species Reactivity | Human|Mouse|Rat |
|---|---|
| Product Format | Liquid. EndoG Antibody is supplied in PBS containing 0.02% sodium azide. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugation | Unconjugated |
| Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
| :: | Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
| Reconstitution and Storage | 2°C to 8°C |
| Immunogen | Anti-EndoG antibody (3035) was raised against a peptide corresponding to 15 amino acids near the amino terminus of human ENDOG. The immunogen is located within amino acids 40-90 of EndoG. |
| Purification | Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
| Concentration | 1 mg/ml |
| Blocking Peptide | For anti-ENDOG (OAPB00172) antibody is Catalog # OEPB02275 |
| Application Info | EndoG antibody can be used for detection of EndoG by Western blot at 1 to 2 ug/ml. HepG2 and 3T3 cell lysates can be used as positive control and a 35 kDa band can be detected. It is human, mouse and rat reactive. (Optimal dilution should be determined by user) |
| Reference | Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature. 2001;412(6842):95-9. ;Parrish J, Li L, Klotz K, Ledwich D, Wang X, Xue D. Mitochondrial endonuclease G is important for apoptosis in C. elegans. Nature. 2001;412(6842):90-4 ;Hengartner MO. Apoptosis. DNA destroyers. Nature. 2001;412(6842):27, 29. ;Widlak P, Li LY, Wang X, Garrard WT. Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. J Biol Chem. 2001;276(51):48404-9. |
| Gene Symbol | ENDOG |
|---|---|
| Gene Full Name | endonuclease G |
| Alias Symbols | endo G;endonuclease G, mitochondrial;mitochondrial endonuclease G. |
| NCBI Gene Id | 2021 |
| Protein Name | Endonuclease G, mitochondrial|endonuclease G, mitochondrial precursor [Homo sapiens] |
| Description of Target | Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA (By similarity). |
| Uniprot ID | Q14249 |
| Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_004426 |
| Nucleotide Accession # | NM_004435.2 |
| Molecular Weight | Predicted: 33kD Observed: 33kD |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ENDOG Antibody (OAPB00172)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "ENDOG Antibody (OAPB00172)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "ENDOG Antibody (OAPB00172)" provided in?
This item is provided in "Liquid. EndoG Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ENDOG Antibody (OAPB00172)"?
This target may also be called "endo G;endonuclease G, mitochondrial;mitochondrial endonuclease G." in publications.
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What is the shipping cost for "ENDOG Antibody (OAPB00172)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ENDOG Antibody (OAPB00172)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ENDOG Antibody (OAPB00172)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 33kD Observed: 33kD".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ENDOG Antibody (OAPB00172)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ENDOG"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ENDOG"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ENDOG"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ENDOG"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ENDOG"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ENDOG"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
