EIF2AK3 Antibody (Phospho-Thr980) (OABF01218)

Catalog No: OABF01218

Size:100UL

Formalin-fixed and paraffin-embedded human lung carcinoma labeled with Anti-Phospho-PERK (Thr980) Polyclonal Antibody, Unconjugated (OABF01218) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37C.

Product Info

• Tested Species Reactivity: Human, Mouse, Rat, Dog, Pig
• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine|Rabbit|Rat
• Product Format: Liquid. Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse PERK around the phosphorylation site of Thr980
• Purification: Protein A Purified
• Concentration: 1 ug/ul
• Target Post-Translational Modification: Thr980
• Application Info: WB: 1:300-1000
  IHC-P: 1:200-400
  ELISA: 1:500~1000
  FC: 1:20~100
  IHC-F: 1:100~500
  Fluorescent (IHC-P): 1:50~200
  Fluorescent (IHC-F):1:50~200
  Fluorescent-ICC: 1:50~200
  Optimal dilutions should be determined by the end user.
• Description:
  View product citations for EIF2AK3 Antibody (Phospho-Thr980) (OABF01218) on CiteAb

Target Info

• Gene Symbol: Eif2ak3
• Gene Full Name: eukaryotic translation initiation factor 2 alpha kinase 3
• Alias Symbols: eukaryotic translation initiation factor 2-alpha kinase 3;pancreatic eIF2-alpha kinase;PE;Pek;Perk;PRKR-like endoplasmic reticulum kinase.
• NCBI Gene Id: 13666
• Protein Name: Eukaryotic translation initiation factor 2-alpha kinase 3
• Description of Target: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability (PubMed:11106749). Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:23921556). Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1) (PubMed:11035797). Involved in control of mitochondrial morphology and function (PubMed:23921556).
• Uniprot ID: Q9Z2B5