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Catalog No: OASG05793
Size:100 uL
Price: $239.00
SKU
OASG05793
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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EIF2AK3 Antibody (Phospho-Thr981) (OASG05793)

Datasheets/ManualsPrintable datasheet for anti-EIF2AK3 (OASG05793) antibody
Product Info
Predicted Species ReactivityHuman, Mouse, Rat
Product FormatLiquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
ClonalityPolyclonal
IsotypeIgG
HostRabbit
ApplicationIHC, IF, WB, ELISA
::Tissue Specificity: Ubiquitous. A high level expression is seen in secretory tissues.
Sequence & Domain Family: The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. Contains 1 protein kinase domain.
Cellular Localization: Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Reconstitution and StorageStore at -20°C. Avoid repeated freeze/thaw cycles.
ImmunogenSynthesized peptide derived from human PERK around the phosphorylation site of T981. Location: 920-1000aa.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Target Post-Translational ModificationOligomerization of the N-terminal ER luminal domain by ER stress promotes PERK trans-autophosphorylation of the C-terminal cytoplasmic kinase domain at multiple residues including Thr-982 on the kinase activation loop (By similarity). Autophosphorylated. Phosphorylated at Tyr-619 following endoplasmic reticulum stress, leading to activate its tyrosine-protein kinase activity. Dephosphorylated by PTPN1/TP1B, leading to inactivate its enzyme activity. N-glycosylated. ADP-ribosylated by PARP16 upon ER stress, which increases kinase activity.
SpecificityPhospho-PERK (T981) Polyclonal Antibody detects endogenous levels of PERK protein only when phosphorylated at T981.
Intended UseFor research use only.
Application InfoIF: 1:50~200
WB: 1:500~2000
IHC: 1:100~300
ELISA: 1:40000
Gene SymbolEIF2AK3
Gene Full Nameeukaryotic translation initiation factor 2-alpha kinase 3
Alias SymbolsPEK, WRS, PERK
NCBI Gene Id9451; 29702
Description of TargetMetabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function. ATP + a protein = ADP + a phosphoprotein. Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase activity induction.
Uniprot IDQ9NZJ5, Q9Z2B5, Q9Z1Z1
Molecular Weight125 kDa
  1. What is the species homology for "EIF2AK3 Antibody (Phospho-Thr981) (OASG05793)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".

  2. How long will it take to receive "EIF2AK3 Antibody (Phospho-Thr981) (OASG05793)"?

    This item is available "Domestic: within 2-3 weeks delivery | International: 2-3 weeks".

  3. What buffer format is "EIF2AK3 Antibody (Phospho-Thr981) (OASG05793)" provided in?

    This item is provided in "Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "EIF2AK3 Antibody (Phospho-Thr981) (OASG05793)"?

    This target may also be called "PEK, WRS, PERK" in publications.

  5. What is the shipping cost for "EIF2AK3 Antibody (Phospho-Thr981) (OASG05793)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "EIF2AK3 Antibody (Phospho-Thr981) (OASG05793)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "EIF2AK3 Antibody (Phospho-Thr981) (OASG05793)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "125 kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "EIF2AK3 Antibody (Phospho-Thr981) (OASG05793)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "EIF2AK3"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "EIF2AK3"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "EIF2AK3"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "EIF2AK3"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "EIF2AK3"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "EIF2AK3"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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