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Catalog No: OKCD03424
Size:96WELLS
Price: $850.00
SKU
OKCD03424
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EGF Chemi-Luminescent ELISA Kit (Mouse) (OKCD03424)

Datasheets/ManualsPrintable datasheet for EGF Chemi-Luminescent ELISA Kit (Mouse) (OKCD03424)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)85-95%84-101%92-101%78-101%
EDTA Plasma (n=5)83-94%89-105%84-103%78-88%
Heparin Plasma (n=5)78-94%99-105%89-103%79-91%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Epidermal Growth Factor (EGF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Epidermal Growth Factor (EGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Epidermal Growth Factor (EGF) level in the sample or standard.
ELISA Kit Range0.69-500pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9388
EDTA Plasma (n=5)80-9588
Heparin Plasma (n=5)78-9889
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epidermal Growth Factor (EGF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epidermal Growth Factor (EGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Egf Microplate96 Wells (12 x 8 Well strips)
Egf Lyophilized Standard2
100X Egf Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma,urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity0.69 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolEgf
Gene Full Nameepidermal growth factor
Alias SymbolsAI790464;pro-epidermal growth factor;Pro-epidermal growth factor precursor (EGF).
NCBI Gene Id13645
Protein NamePro-epidermal growth factor
Description of TargetEGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).
Uniprot IDP01132
Protein Accession #NP_034243
Nucleotide Accession #NM_010113
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