EGF ELISA Kit (Human) : 96 Wells (OKAA00001-96W)

Catalog No: OKAA00001-96W

Size:96 Wells

Standard curve of EGF protein using OKAA00001_96W

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Reproducibility: The coefficient of variation of both intra-assay and inter-assay were less than 10%.
• ELISA Kit Component:

  Components
  1 vial human EGF Standard lyophilized, 20 ng/ml upon reconstitution
  1 vial concentrated Biotin-Conjugate anti-human EGF antibody
  1 vial Streptavidin-HRP solution
  1 bottle Standard /sample Diluent
  1 bottle Biotin-Conjugate antibody Diluent
  1 bottle Streptavidin-HRP Diluent
  1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  1 vial Substrate Solution
  1 vial Stop Solution

• Additional Information: Sample Type: cell culture supernates, serum, and plasma.
  Reagents: REAGENTS
  1. Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human EGF (8×12)
  2. 2 vials human EGF Standard lyophilized, 20 ng/ml upon reconstitution
  3. 2 vials concentrated Biotin-Conjugate anti-human EGF monoclonal antibody
  4. 2 vials Streptavidin-HRP solution,
  5. 1 bottle Standard /sample Diluent
  6. 1 bottle Biotin-Conjugate antibody Diluent
  7. 1 bottle Streptavidin-HRP Diluent
  8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  9. 1 vial Substrate Solution
  10. 1 vial Stop Solution
  11. 3 pieces Adhesive Films
  12. package insert
  NOTE: [96 Tests]
• Sample Type: cell culture, supernates, serum, and plasma
• Sensitivity: 15 pg/ml
• Predicted Homology Based on Immunogen Sequence: Human
• Specificity: This assay recognizes both natural and recombinant human EGF. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
  Recombinant human: G-CSF, GM-CSF, IL-1a, IL-1b, IL-2
  Recombinant mouse: IL-1b, IL-3, IL-4, IL-6, IL-7
  Other proteins: bovine FGF acidic, bovine FGF basic, human PDGF, porcine PDGF, human TGF-b1
• Assay Info: Assay Method: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: EGF
• Gene Full Name: epidermal growth factor
• Alias Symbols: URG, HOMG4
• NCBI Gene Id: 1950
• Protein Name: Pro-epidermal growth factor
• Description of Target: This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
• Uniprot ID: P01133
• Protein Accession #: NP_001171601.1
• Nucleotide Accession #: NM_001178130.2