DNM1L Antibody (Phospho-Ser637) (OASG02244)

Catalog No: OASG02244

Size:100 uL

Western Blot (WB) analysis of JK cells using Phospho-DRP1 (S637) Polyclonal Antibody.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC
  Function: Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues. Also required for mitochondrial fission during mitosis. Required for formation of endocytic vesicles. Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles. Required for programmed necrosis execution. / Isoform 1 and isoform 4 inhibit peroxisomal division when overexpressed. / GTP + H2O = GDP + phosphate. / GTPase activity is increased by binding to phospholipid membranes.
  Cellular Location: Cytoplasm > cytosol / Golgi apparatus / Endomembrane system; Peripheral membrane protein / Mitochondrion outer membrane; Peripheral membrane protein / Peroxisome / Membrane > clathrin-coated pit / Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane
  Tissue Location: Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.
  Sequence and Domain Family: The GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization. / Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. / Contains 1 dynamin-type G (guanine nucleotide-binding) domain. / Contains 1 GED domain.
  
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived at 580-660 amino acids from human DRP1 around the phosphorylation site of S637.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phospho-Ser637
• Specificity: Phospho-DRP1 (S637) Polyclonal Antibody detects endogenous levels of DRP1 protein only when phosphorylated at S637.
• Intended Use: For research use only.
• Application Info: WB 1:500~2000
  IHC 1:100~300
  ELISA 1:10000
  

Target Info

• Gene Symbol: DNM1L
• Gene Full Name: dynamin 1-like
• Alias Symbols: DLP1, DRP1, DVLP, EMPF, OPA5, EMPF1, DYMPLE, HDYNIV
• NCBI Gene Id: 10059; 74006; 114114
• Uniprot ID: O00429, Q8K1M6, O35303
• Molecular Weight: 81 kDa