DEFB103A ELISA Kit (Human) (OKCD08605)

Catalog No: OKCD08605

Size:96WELLS

DEFB103A ELISA Kit (Human) (OKCD08605) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 103A (DEFb103A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 103A (DEFb103A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 103A (DEFb103A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Defensin Beta 103A (DEFb103A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Beta 103A (DEFb103A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Beta 103A (DEFb103A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • DEFB103A Microplate: 96 Wells (12 x 8 Well strips)
  • DEFB103A Lyophilized Standard: 2
  • 100X DEFB103A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 19.54pg/mL

Target Info

• Gene Symbol: DEFB103A|DEFB103B
• Gene Full Name: defensin beta 103A|defensin beta 103B
• Alias Symbols: BD-3;beta-defensin 103;beta-defensin 3;DEFB103;DEFB3;DEFB-3;defensin, beta 103;defensin-like protein;HBD3;HBD-3;HBP3;HBP-3.
• NCBI Gene Id: 414325|55894
• Protein Name: Beta-defensin 103
• Description of Target: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed.
• Uniprot ID: P81534
• Protein Accession #: NP_001075020.1
• Nucleotide Accession #: NM_001081551.3