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Catalog No: OKEH00356
Size:96T
Price: $525.00
SKU
OKEH00356
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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DEFB103A ELISA Kit (Human) : 96 Wells (OKEH00356)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology DEFB103A ELISA Kit (Human) (OKEH00356) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEFB103A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for DEFB103A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DEFB103A captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.9% (n = 20)
Mean Inter-assay CV%: < 9.5% (n = 20)
ELISA Kit Component
ComponentAmount
DEFB103A Microplate96 Wells (12 x 8 Well strips)
DEFB103A Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated DEFB103A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Beta-defensin 103
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDEFB103A, DEFB103B
Gene Full Namedefensin beta 103A, defensin beta 103B
Alias SymbolsBD-3;beta-defensin 103;beta-defensin 3;DEFB103;DEFB3;DEFB-3;defensin, beta 103;defensin-like protein;HBD3;HBD-3;HBP3;HBP-3.
NCBI Gene Id414325, 55894
Protein NameP81534
Description of TargetBeta-defensin 103
Uniprot IDhttps://www.uniprot.org/uniprot/P81534
Protein Accession #NP_001075020.1
Nucleotide Accession #NM_001081551.3
Protein Size (# AA)67
Molecular Weight8 kDa
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