Catalog No: OKCA00967
Size:96 Wells
Price: $675.00
SKU
OKCA00967
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat10195-107
1:29186-97
1:48884-92
1:89590-100
ELISA Kit PrincipleAviva Systems Biology CYP19A1 ELISA Kit (Rat) (OKCA00967) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for CYP19A1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP19A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CYP19A1 captured in well.
ELISA Kit Range18.75 pg/mL-1,200 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)8580-90
EDTA Plasma (n=4)9691-103
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level CYP19A1 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CYP19A1 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
CYP19A1 Microplate96 Wells (12 x 8 Well strips)
CYP19A1 Lyophilized Standard2
100X Biotinylated CYP19A1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity4.68 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCYP19A1
Gene Full NameAromatase
NCBI Gene Id100328545
Protein NameAromatase
Description of TargetCatalyzes the formation of aromatic C18 estrogens from C19 androgens.
Swissprot IdQ29605
Protein Accession #NP_001164392.1
Nucleotide Accession #NM_001170921.2
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CYP19A1.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CYP19A1.
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