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Datasheets/Manuals | Printable datasheet for CYP19A1 Antibody (OABB00443) |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human |
Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Application | Immunohistochemistry|Western blot |
Additional Information | Notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: Aromatase is an enzyme responsible for a key step in the biosynthesis of estrogens. It is a member of the cytochrome P450 superfamily, which are monooxygenases that catalyze many reactions involved in steroidogenesis. In particular, aromatase is responsible for the aromatization of androgens into estrogens. The CYP19 gene spans at least 70 kb of genomic DNA and contains 10 exons. By in situ hybridization, the ARO gene is mapped to 15q21.1. The aromatase enzyme can be found in many tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, and endometrium, as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer. It is an important factor in sexual development. Some bodybuilders taking steroids also take antiaromatase supplements to prevent excess testosterone conversion into estrogens, which can cause gynecomastia. |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Polypeptide |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat |
Reference | 1. Chen, S., Besman, M. J., Sparkes, R. S., Zollman, S., Klisak, I., Mohandas, T., Hall, P. F., Shively, J. E.Human aromatase: cDNA cloning, Southern blot analysis, and assignment of the gene to chromosome 15.DNA 7: 27-38, 1988. 2. Harada, N., Ogawa, H., Shozu, M., Yamada, K., Suhara, K., Nishida, E., Takagi, Y.Biochemical and molecular genetic analyses on placental aromatase (P-450-AROM) deficiency.J. Biol. Chem. 267: 4781-4785, 1992. 3. Zhou, D., Pompon, D., Chen, S.Structure-function studies of human aromatase by site-directed mutagenesis: kinetic properties of mutants pro308-to-phe, tyr361-to-phe, tyr361-to-leu, and phe406-to-arg.Proc. Nat. Acad. Sci. 88: 410-414, 1991. |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Aromatase(CYP19A1) detection. Tested with WB, IHC-P in Human. |
Gene Symbol | CYP19A1 |
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Gene Full Name | cytochrome P450 family 19 subfamily A member 1 |
Alias Symbols | ARO;ARO1;aromatase;CPV1;CYAR;CYP19;CYPXIX;cytochrome P450 19A1;cytochrome P450, family 19, subfamily A, polypeptide 1;cytochrome P450, subfamily XIX (aromatization of androgens);cytochrome P-450AROM;estrogen synthase;estrogen synthetase;flavoprotein-linked monooxygenase;microsomal monooxygenase;P-450AROM. |
NCBI Gene Id | 1588 |
Protein Name | Aromatase |
Description of Target | Catalyzes the formation of aromatic C18 estrogens from C19 androgens. |
Uniprot ID | P11511 |
Protein Accession # | NP_000094.2; NP_112503.1; XP_005254247.1; XP_005254248.1 |
Nucleotide Accession # | NM_000103.3; NM_031226.2; XM_005254190.1; XM_005254191.1 |
Molecular Weight | 57883 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CYP19A1 Antibody (OABB00443)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
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How long will it take to receive "CYP19A1 Antibody (OABB00443)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "CYP19A1 Antibody (OABB00443)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CYP19A1 Antibody (OABB00443)"?
This target may also be called "ARO;ARO1;aromatase;CPV1;CYAR;CYP19;CYPXIX;cytochrome P450 19A1;cytochrome P450, family 19, subfamily A, polypeptide 1;cytochrome P450, subfamily XIX (aromatization of androgens);cytochrome P-450AROM;estrogen synthase;estrogen synthetase;flavoprotein-linked monooxygenase;microsomal monooxygenase;P-450AROM." in publications.
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What is the shipping cost for "CYP19A1 Antibody (OABB00443)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CYP19A1 Antibody (OABB00443)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CYP19A1 Antibody (OABB00443)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "57883 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CYP19A1 Antibody (OABB00443)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CYP19A1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CYP19A1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CYP19A1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CYP19A1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CYP19A1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CYP19A1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.