Catalog No: OKEH03452
Size:96 Wells
Price: $525.00
SKU
OKEH03452
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CYP19A1 ELISA Kit (Mouse) (OKEH03452) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cyp19a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Cyp19a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cyp19a1 captured in well.
ELISA Kit Range0.156 - 10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.4% (n = 20)
Mean Inter-assay CV%: <= 9.0% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Cyp19a1 Microplate96 Wells (12 x 8 Well strips)
Cyp19a1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Cyp19a1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Mouse Aromatase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCYP19A1
Gene Full Namecytochrome P450, family 19, subfamily a, polypeptide 1
Alias SymbolsA, Ar, In, Int, aro, ArKO, Cyp1, Int5, p450, Cyp19, Int-5, p450arom
NCBI Gene Id13075
Protein NameCytochrome P450 19A1
Description of TargetCatalyzes the formation of aromatic C18 estrogens from C19 androgens.
Swissprot IdP28649
Protein Accession #NP_031836.1
Nucleotide Accession #NM_007810.3
Protein Size (# AA)503
Molecular Weight58 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express Cyp19a1.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express Cyp19a1.
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