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Catalog No: OKBB00628
Size:96 Wells
Price: $538.00
Availability: Domestic: within 1 week delivery | International: 1 week
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's rat CXCL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL3 has been precoated onto 96-well plates. Standards(E.coli, R33-S101) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat CXCL3 amount of sample captured in plate.
ELISA Kit Range15.6 pg/ml - 1,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
n 161616242424
Mean (pg/mL)170375602161352593
ELISA Kit Component
96-Well plate Pre-coated with Anti-Rat CXCL3 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Rat CXCL3 Standard2 x 10 ng/tube
100X Biotinylated Anti-Rat CXCL3 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant rat CXCL3
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: E.coli; Immunogen sequence: R33-S101
DescriptionFor quantitative detection of rat CXCL3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolCXCL3
Gene Full Namechemokine (C-X-C motif) ligand 3
Alias SymbolsC-X-C motif chemokine 3, Chemokine (CXC motif) ligand 3, Cinc 2, CINC 2b, Cinc2, CINC2b, CXCL 3, Cxcl3, CXCL3_HUMAN, Cytokine induced neutrophil chemoattractant 2, Dcip1, Dendritic cell inflammatory protein 1, Gm1960, GRO protein gamma, GRO-gamma, GRO-gamma(1-73), GRO-gamma(5-73), GRO3, GRO3 oncogene, GROG, Growth regulated protein gamma, Growth-regulated protein gamma, Macrophage inflammatory protein 2 beta precursor, Macrophage inflammatory protein 2-beta, Melanoma growth stimulatory activity gamma, Member 3, MGSA gamma, MIP 2b, MIP2-beta, MIP2B, SCYB3, Small inducible cytokine subfamily B
NCBI Gene Id171551
Protein NameC-X-C motif chemokine 3
Description of TargetChemokine (C-X-C motif) ligand 3 (CXCL3) is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2 in rat. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.
Uniprot IDQ10746
Protein Accession #NP_612531.1
Nucleotide Accession #NM_138522.1
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