Crp ELISA Kit (Mouse) (OKEH04495)

Catalog No: OKEH04495

Size:96 Wells

Mouse Crp ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Crp ELISA Kit (Mouse) (OKEH04495) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Crp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Crp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Crp captured in well.
• ELISA Kit Range: 31.2 - 2000pg/mL
• ELISA Kit Recovery: <= 5.3%
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-Crp Microplate: 96 Wells (12 x 8 Well Strips)
  • Crp Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Crp Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids
• Sensitivity: 15.6 pg/mL
• Specificity: Natural and recombinant Mouse C-reactive protein
• Publications:

  Toll-like Receptor 4-Independent Effects of Lipopolysaccharide Identified Using Longitudinal Serum Proteomics. J Proteome Res. 19, 1258-1266 (2020). 32037835

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CRP
• Gene Full Name: C-reactive protein, pentraxin-related
• Alias Symbols: AI255847
• NCBI Gene Id: 12944
• Protein Name: C-reactive protein
• Description of Target: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
• Uniprot ID: P14847
• Protein Accession #: NP_031794.3
• Nucleotide Accession #: NM_007768.4
• Protein Size (# AA): 225
• Molecular Weight: 25 KDa