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Catalog No: OKEH04495
Size:96T
Price: $500.00
SKU
OKEH04495
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Crp ELISA Kit (Mouse) (OKEH04495) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Crp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Crp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Crp captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit Recovery<= 5.3%
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-Crp Microplate96 Wells (12 x 8 Well Strips)
Crp Lyophilized Standard2
Sample Diluent1 x 20 mL
Crp Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample Typeserum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids
Sensitivity15.6 pg/mL
SpecificityNatural and recombinant Mouse C-reactive protein
Publications

Toll-like Receptor 4-Independent Effects of Lipopolysaccharide Identified Using Longitudinal Serum Proteomics. J Proteome Res. 19, 1258-1266 (2020). 32037835

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolCrp
Gene Full NameC-reactive protein, pentraxin-related
Alias SymbolsAI255847;C-reactive protein;C-reactive protein, petaxin related.
NCBI Gene Id12944
Protein NameP14847
Description of TargetC-reactive protein
Uniprot IDhttps://www.uniprot.org/uniprot/P14847
Protein Accession #NP_031794.3
Nucleotide Accession #NM_007768.4
Protein Size (# AA)225
Molecular Weight25 KDa
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