COMP Chemi-Luminescent ELISA Kit (Human) (OKCD03392)

Catalog No: OKCD03392

Size:96WELLS

Human COMP ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	87-102%	93-105%	78-93%	78-94%
  EDTA Plasma (n=5)	91-103%	80-103%	81-96%	83-104%
  Heparin Plasma (n=5)	98-105%	97-105%	93-101%	86-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Cartilage Oligomeric Matrix Protein (COMP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cartilage Oligomeric Matrix Protein (COMP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cartilage Oligomeric Matrix Protein (COMP) level in the sample or standard.
• ELISA Kit Range: 0.41-300ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	84-101	93
  EDTA Plasma (n=5)	80-101	94
  Heparin Plasma (n=5)	88-99	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cartilage Oligomeric Matrix Protein (COMP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cartilage Oligomeric Matrix Protein (COMP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • COMP Microplate: 96 Wells (12 x 8 Well strips)
  • COMP Lyophilized Standard: 2
  • 100X COMP Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.41 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: COMP
• Gene Full Name: cartilage oligomeric matrix protein
• Alias Symbols: cartilage oligomeric matrix protein;cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple);CTS2;EDM1;EPD1;MED;PSACH;pseudoachondroplasia (epiphyseal dysplasia 1, multiple);THBS5;thrombospondin-5;TSP5.
• NCBI Gene Id: 1311
• Protein Name: Cartilage oligomeric matrix protein
• Description of Target: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity).
• Uniprot ID: P49747
• Protein Accession #: NP_000086
• Nucleotide Accession #: NM_000095