COMP ELISA Kit (Human) (OKBB00457)

Catalog No: OKBB00457

Size:96 Wells

Human COMP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human COMP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for COMP has been precoated onto 96-well plates. Standards(NS0, Q21-A757) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for COMP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human COMP amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	1.02	3.12	6.19	1.13	3.08	6.23
  SD	0.052	0.131	0.254	0.07	0.166	0.324
  CV%	5.1	4.2	4.1	6.2	5.4	5.2

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human COMP Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human COMP Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human COMP Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human COMP
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q21-A757
• Description: For quantitative detection of human COMP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: COMP
• Gene Full Name: cartilage oligomeric matrix protein
• Alias Symbols: cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple), Cartilage oligomeric matrix protein, Cartilage oligomeric matrix protein precursor, COMP, COMP_HUMAN, EDM 1, EDM1, EPD 1, EPD1, Epiphyseal dysplasia 1, Epiphyseal dysplasia 1 multiple, Epiphyseal dysplasia multiple 1, MED, MGC13181, MGC149768, PSACH, pseudoachondroplasia (epiphyseal dysplasia 1, multiple), Pseudoachondroplasia, THBS 5, THBS5, Thrombospondin 5, Thrombospondin-5, Thrombospondin5, TSP5
• NCBI Gene Id: 1311
• Protein Name: Cartilage oligomeric matrix protein
• Description of Target: Cartilage oligomeric matrix protein is a protein that in humans is encoded by the COMP gene. The sequences of rat and bovine COMP indicate that it is a member of the thrombospondin gene family. By Southern blot analysis of a somatic cell hybrid DNA panel and by isotopic in situ hybridization, human COMP gene was mapped to 19p13.1, and the murine COMP gene was mapped to the central region of mouse chromosome 8 by use of an interspecific backcross mapping panel. COMP is a marker of cartilage turnover.
• Uniprot ID: P49747
• Protein Accession #: NP_000086.2
• Nucleotide Accession #: NM_000095.2