Catalog No: OKCD00679 (Formerly GWB-KBBNA5)
Size:96 Wells
Price: $675.00
SKU
OKCD00679
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva Systems Biology COL4A3 ELISA Kit (Human) (OKCD00679) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COL4A3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for COL4A3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample COL4A3captured in well.
ELISA Kit Range1.56-100 ng/mL
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level COL4A3 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level COL4A3 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
ComponentAmount
Anti-COL4A3 Microplate96 Wells (12 x 8 Well Strips)
COL4A3 Lyophilized Standard2 x 100 ng
100X Biotinylated COL4A3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.55 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Collagen Type IV Alpha 3 (COL4a3). No significant cross-reactivity or interference between Collagen Type IV Alpha 3 (COL4a3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCOL4A3
Gene Full Namecollagen type IV alpha 3 chain
Alias SymbolsATS2, ATS3
NCBI Gene Id1285
Protein NameCollagen alpha-3(IV) chain
Description of TargetType IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity; these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms.
Swissprot IdQ01955
Protein Accession #NP_000082
Nucleotide Accession #NM_000091.4
Protein Size (# AA)1670
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express COL4A3.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express COL4A3.
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