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Datasheets/Manuals | Printable datasheet for anti-COL4A3 (ARP60006_P050) antibody |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit |
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Clonality | Polyclonal |
Host | Rabbit |
Application | WB |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human COL4A3 |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 93%; Dog: 91%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 91%; Pig: 93%; Rabbit: 85%; Rat: 91% |
Peptide Sequence | Synthetic peptide located within the following region: DPGQPGPPGEQGPPGRCIEGPRGAQGLPGLNGLKGQQGRRGKTGPKGDPG |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-COL4A3 (ARP60006_P050) antibody is Catalog # AAP60006 (Previous Catalog # AAPP46159) |
Gene Symbol | COL4A3 |
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Gene Full Name | Collagen, type IV, alpha 3 (Goodpasture antigen) |
Alias Symbols | ATS2, ATS3 |
NCBI Gene Id | 1285 |
Description of Target | Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. There are multiple alternate transcripts that appear to be unique to this human alpha 3 gene and alternate splicing is restricted to the six exons that encode this C-terminal domain. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Some exons of this gene are interspersed with exons of an uncharacterized gene which is on the opposite strand. |
Uniprot ID | E7ENN2 |
Protein Accession # | NP_112730 |
Nucleotide Accession # | NM_031362 |
Protein Size (# AA) | 1637 |
Molecular Weight | 156kDa |
Protein Interactions | VHL; UBC; SERPINE2; OSM; MFAP2; FBLN2; FN1; DCN; CD93; MMP9; APP; SAA1; MATN2; TGFBI; CAMK2B; USH2A; ANTXR2; COL4A3BP; HABP2; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "COL4A3 Antibody - middle region (ARP60006_P050)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit".
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How long will it take to receive "COL4A3 Antibody - middle region (ARP60006_P050)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "COL4A3 Antibody - middle region (ARP60006_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "COL4A3 Antibody - middle region (ARP60006_P050)"?
This target may also be called "ATS2, ATS3" in publications.
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What is the shipping cost for "COL4A3 Antibody - middle region (ARP60006_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "COL4A3 Antibody - middle region (ARP60006_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "COL4A3 Antibody - middle region (ARP60006_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "156kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "COL4A3 Antibody - middle region (ARP60006_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "COL4A3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "COL4A3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "COL4A3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "COL4A3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "COL4A3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "COL4A3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.