CFLAR ELISA Kit (Human) (OKCD09301)

Catalog No: OKCD09301

Size:96WELLS

CFLAR ELISA Kit (Human) (OKCD09301) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CASP8 And FADD Like Apoptosis Regulator (CFLAR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CASP8 And FADD Like Apoptosis Regulator (CFLAR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CASP8 And FADD Like Apoptosis Regulator (CFLAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CASP8 And FADD Like Apoptosis Regulator (CFLAR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CASP8 And FADD Like Apoptosis Regulator (CFLAR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CASP8 And FADD Like Apoptosis Regulator (CFLAR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CFLAR Microplate: 96 Wells (12 x 8 Well strips)
  • CFLAR Lyophilized Standard: 2
  • 100X CFLAR HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.058ng/mL

Target Info

• Gene Symbol: CFLAR
• Gene Full Name: CASP8 and FADD like apoptosis regulator
• Alias Symbols: CASH;CASP8 and FADD-like apoptosis regulator;CASP8AP1;caspase homolog;caspase-eight-related protein;caspase-like apoptosis regulatory protein;caspase-related inducer of apoptosis;Casper;cellular FLICE-like inhibitory protein;cFLIP;c-FLIP;c-FLIPL;c-FLIPR;c-FLIPS;CLARP;FADD-like antiapoptotic molecule 1;FLAME;FLAME1;FLAME-1;FLIP;I-FLICE;inhibitor of FLICE;MACH-related inducer of toxicity;MRIT;testis secretory sperm-binding protein Li 233m;Usurpin;usurpin beta.
• NCBI Gene Id: 8837
• Protein Name: CASP8 and FADD-like apoptosis regulator
• Description of Target: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
• Uniprot ID: O15519
• Protein Accession #: NP_001120655.1
• Nucleotide Accession #: NM_001127183.2